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Chronic Myeloid Leukemia, BCR/ABL Studies and Myelo-Proliferative Syndromes

Identification of genes potentially involved in disease transformation of CML

Leukemia volume 19, pages 998–1004 (2005)Cite this article

Abstract

In patients with chronic myeloid leukemia (CML) who do not reach a (near) complete cytogenetic response, the disease progresses over several years from an indolent, chronic phase into a rapidly fatal blast crisis. Events that are responsible for this transformation process are largely unknown. To identify changes in gene expression that occurred during the course of the disease, we performed cDNA subtraction on sequentially stored peripheral blood mononuclear cell pellets, collected throughout the course of disease of a single CML patient. In total, 32 differentially expressed sequences were identified, of which 27 corresponded to known genes. On quantitative PCR, eight of these genes, YWHAZ, GAS2, IL8, IL6, PBEF1, CCL4, SAT and MMRN, showed comparable differential expression in additional CML patient samples. This set of genes can be considered as a starting point for further research on causes of disease transformation in CML and may lead to new targets in the treatment of resistant CML.

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Authors and Affiliations

  1. Department of Hematology, VU University Medical Center, Amsterdam, The Netherlands

    J J W M Janssen, S M Klaver, Q Waisfisz, G J Schuurhuis & G J Ossenkoppele

  2. Department of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands

    G Pasterkamp & D P V de Kleijn

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  1. J J W M Janssen
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Correspondence to J J W M Janssen.

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Janssen, J., Klaver, S., Waisfisz, Q. et al. Identification of genes potentially involved in disease transformation of CML. Leukemia 19, 998–1004 (2005). https://doi.org/10.1038/sj.leu.2403735

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