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  • Biotechnical Methods Section BTS
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Biotechnical Methods Section (BTS)

BARCODE-ALL: accelerated and cost-effective genetic risk stratification in acute leukemia using spectrally addressable liquid bead microarrays

Leukemia volume 17, pages 1404–1410 (2003)Cite this article

Abstract

An increasing number of risk-stratifying genetic lesions in acute leukemia are being discovered and characterized. To translate this important and increasing volume of information from the research laboratory into effective clinical care, however, new, fast and comprehensive assays are needed. Toward this end, we have developed a two-stage multiplexing assay of broad applicability, which combines multiplex polymerase chain reaction with multiplex detection on spectrally addressable liquid bead microarrays. Using pediatric lymphoblastic leukemia as a model system, we demonstrate that all seven of the fusion transcripts resulting from risk-stratifying chromosomal translocations can be assayed in a single well of a 96-well multiplate with 100% specificity and sensitivity, within 6 h of specimen collection. The assay is automatic and high throughput and represents a significant improvement over previously available assays targeting the same genetic changes. We conclude that user-defined assays that multiplex both target selection and detection may have broad applicability in the management of hematological malignancies.

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Authors and Affiliations

  1. Laboratory of Molecular Diagnostics, University of Massachusetts Medical School and Umass Memorial Health Care, University Campus, Worcester, MA, USA

    J Wallace, Y Zhou, M Reardon & G Pihan

  2. Laboratory of Hematopathology, University of Massachusetts Medical School and Umass Memorial Health Care, University Campus, Worcester, MA, USA

    B Woda & G Pihan

  3. Departments of Pathology, University of Massachusetts Medical School and Umass Memorial Health Care, University Campus, Worcester, MA, USA

    J Wallace, Y Zhou, B Woda & G Pihan

  4. Departments of Pediatrics University of Massachusetts Medical School and Umass Memorial Health Care, University Campus, Worcester, MA, USA

    G N Usmani & P Newburger

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  1. J Wallace
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  2. Y Zhou
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  3. G N Usmani
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  4. M Reardon
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  6. B Woda
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  7. G Pihan
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Wallace, J., Zhou, Y., Usmani, G. et al. BARCODE-ALL: accelerated and cost-effective genetic risk stratification in acute leukemia using spectrally addressable liquid bead microarrays. Leukemia 17, 1404–1410 (2003). https://doi.org/10.1038/sj.leu.2402985

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