Abstract
Translocations involving the immunoglobulin loci are recurring events of B cell oncogenesis. The majority of translocations involve the immunoglobulin heavy chain (IGH) locus, while a minor part involves the immunoglobulin light chain loci consisting of the kappa light chain (IGK) located at 2p11.2 and the lambda light chain (IGL) located at 22q11.2. We characterised BAC clones, spanning the IGK and IGL loci, for detection of illegitimate rearrangements by fluorescence in situ hybridisation (FISH). Within the IGL region we have identified six end sequenced probes (22M5, 1152K19, 2036J16, 3188M21, 3115E23 and 274M7) covering the variable (IGLV) cluster and two probes (165G5 and 31L9) covering the constant (IGLC) cluster. Within the IGK region four probes (969D7, 316G9, 122B6 and 2575M21) have been identified covering the variable (IGKV) cluster, and one probe (1021F11) covering the IGK constant (IGKC) cluster. A series of 24 cell lines of different origin have been analysed for the presence of translocations involving the immunoglobulin light chain loci by dual-colour FISH where the split of the variable cluster and the constant cluster indicated a translocation. Probes established in this study can be used for universal screening of illegitimate rearrangements within the immunoglobulin light chain loci in B cell malignancies.
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Acknowledgements
We thank Dr Mariano Rocchi (RMC) for providing the IGKC and IGLC probes, Prof Gilber Lenoir (IARC) for providing the cell lines; Erik Kjærsgaard, Ulla Høy Davidsen and Dr Charles Medom Hansen for reading the manuscript and Marianne Lodahl for help with sequencing. This work was supported by the Danish Cancer Society and the Danish National Research Foundation.
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Poulsen, T., Silahtaroglu, A., Gisselø, C. et al. Detection of illegitimate rearrangements within the immunoglobulin light chain loci in B cell malignancies using end sequenced probes. Leukemia 16, 2148–2155 (2002). https://doi.org/10.1038/sj.leu.2402648
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DOI: https://doi.org/10.1038/sj.leu.2402648