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New acute myeloid leukemia-derived cell line: MUTZ-8 with 5q−

Leukemia volume 16, pages 1556–1561 (2002)Cite this article

Abstract

The advent of continuous human leukemia–lymphoma cell lines as a rich resource of abundant, accessible and manipulable living cells has contributed significantly to a better understanding of the pathophysiology of hematopoietic tumors. We describe the establishment of the new continuous leukemia cell line MUTZ-8 from a patient with acute myeloid leukemia (AML): MUTZ-8 was derived from the peripheral blood of a 63-year-old woman with AML M4 (25 years after onset of myelodysplastic syndromes, MDS). DNA fingerprinting confirmed authenticity and derivation of the cell line. The immunoprofiling as determined by flow cytometry showed that MUTZ-8 is positive mainly for myeloid but also some monocytic and megakaryocytic markers, whereas it is negative for T cell, B cell and erythroid markers. The cell line is constitutively cytokine-dependent, proliferation requiring externally added cytokines. The cytokine response profiles showed a two- to 10-fold growth stimulation of the cells by various cytokines, whereas other cytokines led to growth inhibition. Cytogenetic analysis confirmed the common clonal derivation of the cell line and the malignant clone predominating at the times of sampling. MUTZ-8 displays a deletion of the 5q31 AML/MDS region effected by a non-reciprocal translocation, t(5;11)(q21;q10). The scientific utility of MUTZ-8 lies (1) in its cluster of pathognomonic cytogenetic alterations including a 5q31 breakpoint and (2) in its absolute cytokine dependency and proliferative response to various cytokines.

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References

  1. Drexler HG, Gignac SM, Minowada J . Hematopoietic Cell Lines. In: Hay RJ, Gazdar A, Park JG (eds) Atlas of Human Tumor Cell Lines Academic Press: Orlando 1994 213–250

    Chapter  Google Scholar 

  2. Drexler HG, Zaborski M, Quentmeier H . Cytokine response profiles of human myeloid factor-dependent leukemia cell lines Leukemia 1997 11: 701–708

    Article  CAS  PubMed  Google Scholar 

  3. Oval J, Taetle R . Factor-dependent human leukemia cell lines: new models for regulation of acute non-lymphocytic leukemia cell growth and differentiation Blood Rev 1990 4: 270–279

    Article  CAS  PubMed  Google Scholar 

  4. Drexler HG, MacLeod RAF, Borkhardt A, Janssen JWG . Recurrent chromosomal translocations and fusion genes in leukemia–lymphoma cell lines Leukemia 1995 9: 480–500

    CAS  PubMed  Google Scholar 

  5. Drexler HG (ed). The Leukemia–Lymphoma Cell Line FactsBook Academic Press: San Diego 2000

    Google Scholar 

  6. Drexler HG, Matsuo Y . Guidelines for the characterization and publication of human malignant hematopoietic cell lines Leukemia 1999 13: 835–842

    Article  CAS  PubMed  Google Scholar 

  7. Drexler HG, Uphoff CC . Contamination of cell culture, mycoplasma. In: Spier E et al (eds) The Encyclopedia of Cell Technology Wiley: New York 2000 609–627

    Google Scholar 

  8. Drexler HG, Dirks WG, MacLeod RAF . False human hematopoietic cell lines: cross-contaminations and misinterpretations Leukemia 1999 13: 1601–1607

    Article  CAS  PubMed  Google Scholar 

  9. Drexler HG, Dirks WG, MacLeod RAF, Quentmeier H, Steube KG, Uphoff CC (eds). DSMZ Catalogue of Human and Animal Cell Lines, 8th edn DSMZ: Braunschweig, Germany 2001

    Google Scholar 

  10. Hu ZB, Ma W, Zaborski M, MacLeod RAF, Quentmeier H, Drexler HG . Establishment and characterization of two novel cytokine-responsive acute myeloid and monocytic leukemia cell lines, MUTZ-2 and MUTZ-3 Leukemia 1996 10: 1025–1040

    CAS  PubMed  Google Scholar 

  11. Matsuo Y, MacLeod RAF, Uphoff CC, Drexler HG, Nishizaki C, Katayama Y, Kimura G, Fujii N, Omoto E, Harada M, Orita K . Two acute monocytic leukemia (AML-M5a) cell lines (MOLM-13 and MOLM-14) with interclonal phenotypic heterogeneity showing MLL-AF9 fusion resulting from an occult chromosome insertion, ins(11;9)(q23;p22p23) Leukemia 1997 11: 1469–1477

    Article  CAS  PubMed  Google Scholar 

  12. MacLeod RA, Spitzer D, Bar-Am I, Sylvester JE, Kaufmann M, Wernich A, Drexler HG . Karyotypic dissection of Hodgkin's disease cell lines reveals ectopic subtelomeres and ribosomal DNA at sites of multiple jumping translocations and genomic amplification Leukemia 2000 14: 1803–1814

    Article  CAS  PubMed  Google Scholar 

  13. MacLeod RA, Dirks WG, Matsuo Y, Kaufmann M, Milch H, Drexler HG . Widespread intraspecies cross-contamination of human tumor cell lines arising at source Int J Cancer 1999 83: 555–563

    Article  CAS  PubMed  Google Scholar 

  14. Le Beau MM, Albain KS, Larson RA, Vardiman JW, Davis EM, Blough RR, Golomb HM, Rowley JD . Clinical and cytogenetic correlations in 63 patients with therapy-related myelodysplastic syndromes and acute nonlymphocytic leukemia: further evidence for characteristic abnormalities of chromosomes no. 5 and 7 J Clin Oncol 1986 4: 325–345

    Article  CAS  PubMed  Google Scholar 

  15. Boultwood J, Lewis S, Wainscoat JS . The 5q− syndrome Blood 1994 84: 3253–3260

    CAS  PubMed  Google Scholar 

  16. Andersen MK, Pedersen-Bjergaard J . Increased frequency of dicentric chromosomes in therapy-related MDS and AML compared to de novo disease is significantly related to previous treatment with alkylating agents and suggests a specific susceptibility to chromosome breakage at the centromere Leukemia 2000 14: 105–111

    Article  CAS  PubMed  Google Scholar 

  17. Christiansen DH, Andersen MK, Pedersen-Bjergaard J . Mutations with loss of heterozygosity of p53 are common in therapy-related myelodysplasia and acute myeloid leukemia after exposure to alkylating agents and significantly associated with deletion or loss of 5q, a complex karyotype, and a poor prognosis J Clin Oncol 2001 19: 1405–1413

    Article  CAS  PubMed  Google Scholar 

  18. Johansson B, Mertens F, Heim S, Kristoffersson U, Mitelman F . Cytogenetics of secondary myelodysplasia (sMDS) and acute nonlymphocytic leukemia (sANLL) Eur J Haematol 1991 47: 1727

    Google Scholar 

  19. de Souza Fernandez T, Ornellas MH, Otero de Carvalho L, Tabak D, Abdelhay E . Chromosomal alterations associated with evolution from myelodysplastic syndrome to acute myeloid leukemia Leukemia Res 2000 24: 839–848

    Article  CAS  Google Scholar 

  20. Steube KG, Gignac SM, Hu ZB, Teepe D, Harms D, Kabisch H, Gaedicke G, Hansen-Hagge T, MacLeod RA, Quentmeier H, Drexler HG . In vitro culture studies of childhood myelodysplastic syndrome: establishment of the cell line MUTZ-1 Leuk Lymphoma 1997 25: 345–363

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We thank Drs W Dirks and C Uphoff for performing DNA fingerprinting and viral/mycoplasmal analysis.

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Authors and Affiliations

  1. Department of Medicine, University of Illinois at Chicago, Chicago, IL, USA

    Z-B Hu

  2. DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany

    RAF MacLeod, C Meyer, H Quentmeier & HG Drexler

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  1. Z-B Hu
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Hu, ZB., MacLeod, R., Meyer, C. et al. New acute myeloid leukemia-derived cell line: MUTZ-8 with 5q−. Leukemia 16, 1556–1561 (2002). https://doi.org/10.1038/sj.leu.2402601

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