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Establishment and comprehensive analysis of a new human transformed follicular lymphoma B cell line, Tat-1

Leukemia volume 16, pages 276–283 (2002)Cite this article

Abstract

A spontaneously EBV transformed follicular lymphoma (FL) cell line, Tat-1, was established from the lymph node biopsy specimen of a patient with B cell FL, grade 1 in transformation to high grade disease. Tat-1 cells expressed lymphoid markers and developed tumor masses in immunodeficient mice. Bcl-2, Bcl-XL, Bax and p53 protein expression was revealed by Western blotting. Flow cytometric analysis confirmed P-gp expression. Cytogenetically, the Tat-1 cell line showed identical chromosomal alterations to that of the initial biopsy specimen, among which the most notable were the t(14;18) typical of FL and additional abnormalities involving chromosomes 1, 8 and 13. Multicolor FISH analysis delineated all abnormalities, including a t(1p;8q), a der(8)(8q24::14q32::18q21) and a der(13)(13q32::8q24::14q32::18q21). Further FISH investigations using a locus-specific probe cocktail containing c-myc, IgH and bcl-2 revealed fusion of these three loci on the derivatives 8 and 13, in addition to the derivative 14 IgH/bcl-2 fusion and an extra copy of c-myc on derivative chromosome 1. These results demonstrate an additional example of the deregulation of bcl-2 and c-myc expression through recombination with a single IgH enhancer region. The unusual molecular features of the Tat-1 cell line render it a unique tool for studies focused on cytogenetic alterations, expression of multidrug resistance phenotype and expression of anti-apoptotic proteins in FL.

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Acknowledgements

This work was supported by The National Cancer Institute of Canada with funds from the Canadian Cancer Society. We are very grateful to Don Philips, Kees Pot and Visia Dragovska for technical assistance in flow cytometric analysis, Dana Masin for animal work assistance and Dr Chris Williams for clinical care of the patient.

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Authors and Affiliations

  1. Department of Advanced Therapeutics, Vancouver Cancer Centre, British Columbia Cancer Agency, and the University of British Columbia, Vancouver, BC, Canada

    T Denyssevych, S Knesevich, M Robichaud, C Salski & LD Mayer

  2. Department of Pathology and Laboratory Medicine, Vancouver Cancer Centre, British Columbia Cancer Agency, and the University of British Columbia, Vancouver, BC, Canada

    VS Lestou, R Tan, RD Gascoyne & DE Horsman

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  1. T Denyssevych
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Denyssevych, T., Lestou, V., Knesevich, S. et al. Establishment and comprehensive analysis of a new human transformed follicular lymphoma B cell line, Tat-1. Leukemia 16, 276–283 (2002). https://doi.org/10.1038/sj.leu.2402372

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