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P15INK4B gene methylation and expression in normal, myelodysplastic, and acute myelogenous leukemia cells and in the marrow cells of cured lymphoma patients

Leukemia volume 15, pages 1589–1595 (2001)Cite this article

Abstract

P15INK4B methylation and expression was studied in bone marrow cells obtained from normal individuals, from patients who had been cured of lymphoma, and from patients with either MDS or AML. The level of p15 methylation was very low in normal BM cells and in CD34+ and CD34− subpopulations (0–6.5%; med, = 2.5%). P15INK4B transcripts were present in each of these cell populations. In contrast, methylation was the usual situation in MDS and AML marrows. The presence of methylation of the p15INK4B gene did not always indicate an absence of expression nor was expression always present if methylation was absent. P15INK4B methylation was studied in the marrows of nine patients (one studied twice) who had been cured of lymphoma and in whom hemopoiesis was believed to be normal. Increased methylaton was present in all 10 marrows. These data indicate that p15INK4B methylation is likely to be a very early event in the development of the secondary hematologic disorders.

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Acknowledgements

This work was supported by the National Cancer Institute grant 1-PO-1CA75606-04.

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  1. Rush Cancer Institute, 1725 W. Harrison Street, Suite 809 Professional Building I, Chicago, Illinois 60612, USA

    HD Preisler, B Li, H Chen, L Fisher, J Nayini, A Raza, S Creech & P Venugopal

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  1. HD Preisler
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Preisler, H., Li, B., Chen, H. et al. P15INK4B gene methylation and expression in normal, myelodysplastic, and acute myelogenous leukemia cells and in the marrow cells of cured lymphoma patients. Leukemia 15, 1589–1595 (2001). https://doi.org/10.1038/sj.leu.2402211

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