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T cell activation following infection of primary follicle center lymphoma B cells with adenovirus encoding CD154

Leukemia volume 15, pages 1451–1457 (2001)Cite this article

Abstract

Purified, high-titer adenovirus encoding murine CD154 (Ad-CD154) or human CD154 (Ad-hCD154) was used to infect lymph node cells isolated from patients with follicle center lymphoma. Infection of lymphoma B cells with Ad-CD154 at a multiplicity of infection (MOI) ratio of 100 or higher resulted in high-level transgene expression. Additionally, upon infection of lymphoma B cells, only Ad-CD154 resulted in surface expression of CD154, despite similar, high-level expression of either human or mouse CD154 by HeLa cells infected with Ad-hCD154 or Ad-CD154, respectively. Moreover, infection of lymphoma B cells with Ad-CD154, but not Ad-hCD154 or adenovirus encoding Eschericheria coli beta-galactosidase (Ad-LacZ), induced the neoplastic B cells to express higher levels of immune co-stimulatory molecules that are required for proficient presentation of antigen to T cells. Consistent with this, we found that Ad-CD154 infected lymphoma B cells could stimulate T cells to proliferate or produce interferon-gamma in allogeneic or autologous mixed lymphocyte interactions. We conclude that lymphoma B cells can be infected with Ad-CD154 and that this significantly enhances their recognition by allogeneic or autologous T cells. As such, Ad-CD154-transduced lymphoma B cells may have potential for the active immune therapy of patients with follicle center lymphoma.

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Acknowledgements

These studies funded in part by PO1-CA81534, PO1-CA34233, and PO1-CA72103 from the NIH

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Authors and Affiliations

  1. Immunogenex, Inc., La Jolla, CA, USA

    MJ Cantwell, WG Wierda, IS Lossos, R Levy & TJ Kipps

  2. Division of Oncology, Stanford University Medical School, Stanford, CA, USA

    MJ Cantwell, WG Wierda, IS Lossos, R Levy & TJ Kipps

  3. Division of Hematology/Oncology, UCSD School of Medicine, La Jolla, CA, USA

    MJ Cantwell, WG Wierda, IS Lossos & R Levy

  4. Division of Hematology/Oncology, Department of Medicine, University of Californai-San Diego, 9500 Gilman Dr., La Jolla, CA, 92093-0663, USA

    TJ Kipps

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Cantwell, M., Wierda, W., Lossos, I. et al. T cell activation following infection of primary follicle center lymphoma B cells with adenovirus encoding CD154. Leukemia 15, 1451–1457 (2001). https://doi.org/10.1038/sj.leu.2402208

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