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Myeloma

Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM)

Leukemia volume 15, pages 1268–1273 (2001)Cite this article

Abstract

In order to investigate the frequency of HHV-8 in MM patients from another geographic location, we obtained fresh bone marrow (BM) biopsies from Turkish patients with MM (n = 21), monoclonal gammopathy of undetermined significance (MGUS) (n = 2), plasmacytoma (n = 1) with BM plasma cell infiltration, various hematological disorders (n = 6), and five healthy Turkish controls. The frequency of HHV-8 was analyzed by polymerase chain reaction (PCR) in two independent laboratories in the USA and in Turkey. Using fresh BM biopsies, 17/21 MM patients were positive for HHV-8 whereas all five healthy controls, and six patients with other hematological disorders were negative. Two patients with MGUS, and one patient with a solitary plasmacytoma were also negative. The data from the two laboratories were completely concordant. Also using primer pairs for v IRF and v IL-8R confirmed the results observed with the KS330233 primers. Furthermore, sequence analysis demonstrated a C3 strain pattern in the ORF26 region which was also found in MM patients from the US. Thus, HHV-8 is present in the majority of Turkish MM patients, and the absence of the virus in healthy controls further supports its role in the pathogenesis of MM.

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Acknowledgements

M Beksac is a member and is partially supported by the Turkish Academy of Sciences.

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Authors and Affiliations

  1. Department of Hematology, Ibni Sina Hospital, Ankara University School of Medicine, Ankara, Turkey

    M Beksac, M Arat, N Konuk & H Koc

  2. Department of Molecular Biology and Genetics, Bilkent University, Ankara, Turkey

    C Akyerli & T Ozcelik

  3. Cedars Sinai Medical Center, Jonsson Comprehensive Cancer Center, UCLA School of Medicine, Los Angeles, CA, USA

    M Ma, M DerDanielian, L Zhang, J Liu, R Vescio & JR Berenson

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Beksac, M., Ma, M., Akyerli, C. et al. Frequent demonstration of human herpesvirus 8 (HHV-8) in bone marrow biopsy samples from Turkish patients with multiple myeloma (MM). Leukemia 15, 1268–1273 (2001). https://doi.org/10.1038/sj.leu.2402190

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