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Appendix: Method in Focus

Quantitative analysis of chimerism after allogeneic stem cell transplantation using multiplex PCR amplification of short tandem repeat markers and fluorescence detection

Leukemia volume 15, pages 303–306 (2001)Cite this article

The method is based on a commercially available multiplex STR (short tandem repeat) PCR kit originally designed for forensic purposes (genetic fingerprinting). Nine different STR markers and the amelogenin locus are co-amplified in a single reaction. Separation of the PCR products and fluorescence detection are performed either on gel-based sequencers or capillary electrophoresis instruments. Calculation of donor chimerism is based on the ratio of informative donor and recipient signals.

It is assumed that the user is familiar with the sequencing instrument. Reading of the AmpFlSTR Profiler PCR Amplification Kit user manual is strongly recommended prior to starting the assay.

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Quantitative analysis of chimerism after allogeneic stem cell transplantation using multiplex PCR amplification of short tandem repeat markers and fluorescence detection. Leukemia 15, 303–306 (2001). https://doi.org/10.1038/sj.leu.2402008

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