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  • Biotechnical Methods Section BTS
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Biotechnical Methods Section (BTS): Special Technical Report

A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method

Leukemia volume 11, pages 1588–1593 (1997)Cite this article

Abstract

A novel variant of the chimerical MLL-AF10 mRNA transcript was detected in a pediatric patient with acute myeloid leukemia (AML) by a new asymmetric reverse-transcription polymerase chain reaction (ART-PCR) method. Sequence analysis of the fusion region on the amplified cDNA fragment showed an in-frame joining of exon e5 of the MLL gene and position 1931 of the cDNA sequence of the AF10 gene, giving rise to a new MLL-AF10 transcript. The presence of the new chimerical mRNA product in a sample from the patient was confirmed by classical RT-PCR.

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Authors and Affiliations

  1. Institute of Molecular and Structural Biology, Aarhus University, Denmark

    B Hjorth-Sørensen, N Pallisgaard, M Grönholm & P Jørgensen

  2. Department of Haematology, Aarhus University Hospital, Denmark

    P Hokland

  3. Department of Pediatrics, University Hospital of Aarhus at Skejby, Denmark

    N Clausen

Authors
  1. B Hjorth-Sørensen
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  2. N Pallisgaard
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  3. M Grönholm
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  4. P Hokland
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  5. N Clausen
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  6. P Jørgensen
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Hjorth-Sørensen, B., Pallisgaard, N., Grönholm, M. et al. A novel MLL-AF10 fusion mRNA variant in a patient with acute myeloid leukemia detected by a new asymmetric reverse transcription PCR method. Leukemia 11, 1588–1593 (1997). https://doi.org/10.1038/sj.leu.2400758

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  • DOI: https://doi.org/10.1038/sj.leu.2400758

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