Abstract
THE growth rate of cells isolated from higher organisms depends largely on population density. At low cell densities, cells have a low probability of growing in usual culture conditions (reviewed in ref. 1). Cell growth can be sustained in sparse cultures using X-irradiated feeder layers or conditioned medium. Feeder layers are thought to enhance growth by producing “conditioned factor”, which seems to be restricted to the vicinity of feeder cells2–4, and is hardly diffusible into culture medium. Conditioned factor was supposed to be a kind of pericellular substance such as microexudate, which was detected by Rosenberg5 by using ellipsometry. While similar substances have been observed for several types of cultured cells6,7, little has been known about their chemical, morphological and biological properties. We have examined the microexudate carpet by electron microscopy after improving the sectioning technique for monolayer cultures of chick embryo cells.
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YAOI, Y., KANASEKI, T. Role of Microexudate Carpet in Cell Division. Nature 237, 283–285 (1972). https://doi.org/10.1038/237283a0
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DOI: https://doi.org/10.1038/237283a0
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