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Human Blastocysts grown in Culture

Nature volume 229, pages 132–133 (1971)Cite this article

Abstract

WE have already described the culture of cleaving human embryos to the sixteen celled stage1, and we now wish to give details of a few embryos that have developed much further, including two that reached fully developed blastocysts. Methods were similar to those described before. Preovulatory oocytes recovered by laparoscopy2 were fertilized in Bavister's medium3, and transferred after 12–15 h into Ham's F 10 supplemented with human or foetal calf serum. Preliminary details of this work have been presented elsewhere4.

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References

  1. Edwards, R. G., Steptoe, P. C., and Purdy, J. M., Nature, 227, 1307 (1970).

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  2. Steptoe, P. C., and Edwards, R. G., Lancet, i, 683 (1970).

  3. Bavister, B. D., J. Reprod. Fert., 18, 544 (1969).

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  4. Edwards, R. G., Harold C. Mack Symposium on the Biology of Fertilization and Implantation, Detroit, October 1970 (in the press).

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  5. Tarkowski, A. K., Cytogenetics, 5, 394 (1966).

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  6. Amoroso, E. C., Memoirs of the Society for Endocrinology, 6, 50 (1959).

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Authors and Affiliations

  1. Oldham General Hospital, Lancashire, and Physiological Laboratory, University of Cambridge,

    P. C. STEPTOE, R. G. EDWARDS & J. M. PURDY

Authors
  1. P. C. STEPTOE
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  2. R. G. EDWARDS
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  3. J. M. PURDY
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STEPTOE, P., EDWARDS, R. & PURDY, J. Human Blastocysts grown in Culture. Nature 229, 132–133 (1971). https://doi.org/10.1038/229132a0

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