Abstract
MULTIPLE forms of RNA polymerase activity have been isolated from developing sea-urchin embryos, rat liver nuclei and calf thymus1–3. The molecular basis of this heterogeneity is at present unknown but, whatever its basis, the crucial question seems to be whether different enzymes copy different genes. We report here the isolation and purification of two different RNA polymerases from Xenopus oocytes; only one of them is localized in the nucleolus. On the basis of localization, binding to ribosomal DNA, and response to inhibitors, we conclude that the nucleolar RNA polymerase is responsible for ribosomal RNA synthesis in vivo.
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References
Roeder, R. G., and Rutter, W. J., Nature, 224, 234 (1969).
Kedinger, C., Gniazdowski, M., Mandel, jun., J. L., Gissinger, F., and Chambon, P., Biochem. Biophys. Res. Commun., 38, 165 (1970).
Jacob, S. T., Sajdel, E. M., and Munro, H. N., Biochem. Biophys. Res. Commun., 32, 831 (1968).
Birnstiel, M., in Cell Differentiation (Ciba Foundation Symp.), 178 (Churchill, London, 1967).
Reeder, R., and Brown, D. D., Proc. Lepetit Colloquium on RNA Polymerase, Florence, 249 (North-Holland, Amsterdam, 1969).
Jones, O. W., and Berg, P., J. Mol. Biol., 22, 199 (1966).
Fiume, L., and Stirpe, F., Biochim. Biophys. Acta, 123, 643 (1969).
Seifart, H. K., Proc. Lepetit Colloquium on RNA Polymerase, Florence, 233 (North-Holland, Amsterdam, 1969).
Chambon, P., Ramuz, M., Mandel, P., and Doly, J., Biochim. Biophys. Acta, 157, 504 (1968).
Jacob, S. T., Sajdel, E. M., and Munro, H. N., Biochem. Biophys. Res. Commun., 38, 765 (1970).
Roeder, R. G., and Rutter, W. J., Proc. US Nat. Acad. Sci., 65, 675 (1970).
Miller, J. O., and Beatty, P. R., Science, 164, 955 (1969).
Dawid, I., Proc. US Nat. Acad. Sci., 56, 269 (1966).
Davidson, E. H., and Mirsky, A. E., Brookhaven Symp. Biol., 18, 77 (1965).
Brown, D. D., and David, I. B., Science, 160, 272 (1968).
Thiry, L., and Lancini, G., Nature, 227, 1048 (1970).
Birnstiel, M., Speirs, J., Purdom, I., Iones, K., and Loening, V. E., Nature, 219, 454 (1968).
Brown, D. D., and Littna, E., J. Mol. Biol., 8, 688 (1964).
Crippa, M., and Tocchini-Valentini, G. P., Nature, 226, 1243 (1970).
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TOCCHINI-VALENTINI, G., CRIPPA, M. Ribosomal RNA Synthesis and RNA Polymerase. Nature 228, 993–995 (1970). https://doi.org/10.1038/228993a0
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DOI: https://doi.org/10.1038/228993a0
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