Abstract
THE cell fusion technique of Harris1,2, involving the use of ultraviolet inactivated Sendai virus3, can be used to fuse differentiated cells with each other and with nondifferentiated cells. In some cases the hybrid cells form synkaryons capable of cell proliferation, but although some express surface antigens characteristic of the component cell nuclei4, none has yet been found to produce the “luxury” molecules5 characteristic of highly differentiated cells. This may be because conditions which favour cell proliferation are antagonistic to cell differentiation. In vitro differentiation of normal cells is usually observed only in very special conditions, of which cell concentration, cell to cell interactions and preconditioning of the medium or the supporting surface may be critical.
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References
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CARLSSON, SA., SAVAGE, R. & RINGERTZ, N. Behaviour of Differentiated Hen Nuclei in the Cytoplasm of Rat Myoblasts and Myotubes. Nature 228, 869–871 (1970). https://doi.org/10.1038/228869a0
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DOI: https://doi.org/10.1038/228869a0
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