Abstract
THE presence of reovirus RNA transcriptase within the virion has facilitated studies on the mechanism of mRNA synthesis in vitro1,2. Subviral particles prepared from extracts of reovirus infected L-929 cells have provided stable and highly active RNA transcriptase preparations3. The particulate enzyme efficiently synthesizes in vitro all ten species of the reovirus-specific single-stranded mRNAs that correspond to the ten segments of the double-stranded RNA genome3,5. The same transcription mechanism seems to apply in vivo and in vitro as suggested by (a) the correlation in size and distribution of the messenger species3–8 and (b) the complementarity of the corresponding mRNAs to the same template strand (unpublished data of D. Kyner).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Borsa, Y., and Graham, A. F., Biochem. Biophys. Res. Commun., 33, 895 (1968).
Shatkin, A. J., and Sipe, J. D., Proc. US Nat. Acad. Sci., 61, 1462 (1968).
Levin, D. H., Mendelsohn, N., Schonberg, M., Klett, H., Acs, G., Silverstein, S., and Kapuler, A., Proc. US Nat. Acad. Sci. (in the press).
Skehel, J. J., and Joklik, W. K., Virology, 39, 822 (1969).
Banerjee, A. K., and Shatkin, A. J., J. Virol. (in the press).
Shatkin, A. J., and Rada, B., J. Virol., 1, 24 (1967).
Watanabe, Y., Prevec, L., and Graham, A. F., Proc. US Nat. Acad. Sci., 58, 1040 (1967).
Gomatos, P. J., J. Mol. Biol., 37, 423 (1968).
Banerjee, A. K., Eoyang, L., Hori, K., and August, J. T., Proc. US Nat. Acad. Sci. 57, 986 (1967).
Spiegelman, S., Pace, N. R., Mills, D. R., Levisohn, R., Eikhorn, T. S., Taylor, M. M., Peterson, R. L., and Bishop, D. H. L., Cold Spring Harbor Symp. Quant. Biol., 33, 101 (1968).
Billetei, M. A., Dahlberg, J. E., Goodman, H. M., Hindley, J., and Weissmann, C., Nature, 224, 1083 (1969).
Roblin, R., J. Mol. Biol., 21, 51 (1968).
Glitz, D., Biochemistry, 7, 927 (1968).
Maitra, U., and Hurwitz, J., Proc. US Nat. Acad. Sci., 54, 815 (1965).
Bremer, H., Konrad, M. W., Gaines, K., and Stent, G. S., J. Mol. Biol., 13, 540 (1965).
Franklin, R. M., Proc. US Nat. Acad. Sci., 55, 1504 (1966).
Robertson, H. D., Webster, R. E., and Zinder, N. D., J. Biol. Chem., 243, 82 (1968).
Shatkin, A. K., Sipe, J. D., and Loh, P., J. Virol., 2, 986 (1968).
Zillig, W., Schactschabel, D., and Krone, W., Z. Physiol. Chem., 318, 100 (1960).
Singer, M. F., and Cantoni, G. L., Biochim. Biophys. Acta, 39, 182 (1960).
Takanami, M., Cold Spring Harbor Symp. Quant. Biol., 31, 611 (1966).
Takanami, M., J. Mol. Biol., 23, 135 (1967).
RajBhandary, U. L., Young, R. J., and Khorana, H. G., J. Biol. Chem., 239, 3875 (1964).
Maitra, U., Novogrodsky, A., Baltimore, D., and Hurwitz, J., Biochem. Biophys. Res. Commun., 18, 801 (1965).
Kapuler, A., Mendelsohn, N., Klett, H., and Acs, G., Nature, 225, 1209 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
LEVIN, D., ACS, G. & SILVEBSTEIN, S. Chain Initiation by Reovirus RNA Transcriptase in vitro. Nature 227, 603–604 (1970). https://doi.org/10.1038/227603a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/227603a0
This article is cited by
-
5′-Terminal Nucleotide Sequence of Reovirus mRNA synthesized in vitro
Nature New Biology (1972)
-
Initiation of Reovirus mRNA Synthesis in vitro
Nature New Biology (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.