Abstract
THE localized haemolysis-in-gel (LHG) assay1, originally applied to the detection and enumeration of cells producing antibodies to erythrocyte cell wall antigens, has been extended to the detection of cells forming antibodies to hapten2–7 and protein antigens8,9. In these experiments the antigens or haptens were bound to the erythrocyte by direct chemical reaction. From our experience, and that of others, the main drawbacks of such a chemical approach for the attachment of ligands to a living cell are as follows: (1) direct chemical modification of the erythrocyte causes damage to the cell membrane resulting in cellular fragility and tendency to haemolyse6; (2) because crythrocytes must be sensitized anew each day, fresh reagents and large amounts of materials (in the case of protein antigens) are required on each day of the experiment4,8; (3) the extent of modification may vary between preparations, and the degree of substitution is difficult to measure, resulting in irreproducibility of results.
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STRAUSBAUCH, P., SULICA, A. & GIVOL, D. General Method for the Detection of Cells producing Antibodies against Haptens and Proteins. Nature 227, 68–69 (1970). https://doi.org/10.1038/227068a0
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DOI: https://doi.org/10.1038/227068a0
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