Abstract
L-ASPARAGINASE (EC 3.5.1.1), which hydrolyses the amide group of asparagine, inhibits certain neoplasms1–4 and is used for treating human acute leukaemias5,6. Although asparaginase sequentially inhibits protein, RNA and DNA synthesis7, its cellular action is undefined. We have investigated the effects of asparaginase on the synthesis of carbohydrate–amino-acid linkages in glycoproteins which involve the amide group of asparagine and N-acetyl-glucosamine residues8. Our preparation of L-asparaginase (from Escherichia coli, 321–363 U/mg protein) was free of contaminating enzymatic activity, although it was slightly active (2.1 per cent of the activity towards asparagine) with glutamine as substrate. The preparation was free of glycosidase and proteolytic activities9,10.
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References
Boyse, E. A., Old, L. J., Campbill, H. A., and Mashburn, L. T., J. Exp. Med., 125, 17 (1967).
Mashburn, L. T., and Gordon, C. S., Cancer Res., 28, 961 (1968).
Broome, J. D., J. Exp. Med., 127, 1055 (1968).
Sugiura, K., Cancer Chemotherap. Rep., 53, 189 (1969).
Oettgen, H. F., and Schulten, H. K., Klin. Wschr., 47, 65 (1965).
Adamson, R. H., and Fabro, S., Cancer Chemotherap. Rep., 52, 617 (1968).
Ellem, K., Fabrezio, A., and Jackson, L., Proc. Amer. Assoc. Cancer Res., 10, 21 (1969).
Spiro, R. G., New England J. Med., 281, 991 (1969).
Bosmann, H. B., Exp. Cell Res., 54, 217 (1969).
Bosmann, H. B., and Merritt, W. D., Physiol. Chem. Phys., 1, 555 (1969).
Fischer, G. A., and Sartorelli, A. C., Methods Med. Res., 10, 247 (1964).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. Biol. Chem., 193, 265 (1951).
Spiro, R. G., J. Biol. Chem., 235, 2860 (1960).
Katzman, R. L., and Eylar, E. H., Biochem. Biophys. Res. Commun., 23, 769 (1966).
Katzman, R. L., and Eylar, E. H., Arch. Biochem. Biophys., 117, 623 (1966).
Bosmann, H. B., Hagopian, A., and Eylar, E. H., Arch. Biochem. Biophys., 128, 470 (1968).
Bosmann, H. B., and Martin, S. S., Science, 164, 190 (1969).
Bosmann, H. B., Europ. J. Biochem. (in the press).
Bosmann, H. B., Hagopian, A., and Eylar, E. H., Arch. Biochem. Biophys., 130, 573 (1969).
Bosmann, H. B., Hagopian, A., and Eylar, E. H., J. Cell Physiol., 72, 81 (1968).
Berenbaum, M. C., Nature, 225, 550 (1970).
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BOSMANN, H., KESSEL, D. Inhibition of Glycoprotein Synthesis in L5178Y Mouse Leukaemic Cells by L-Asparaginase in vitro. Nature 226, 850–851 (1970). https://doi.org/10.1038/226850a0
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DOI: https://doi.org/10.1038/226850a0
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