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Fast Spectrophotometric Measurement of H+ Changes in Chromatium Chromatophores activated by a Liquid Dye Laser

Nature volume 225, pages 705–708 (1970)Cite this article

Abstract

The fast changes in hydrogen ion concentration which follow photo-activation of the bacteriochlorophyll complex of Chromatium have been measured by observing absorbance changes of the pH indicator bromcresol purple using a ruby-pumped dye laser. This acts as an optical frequency converter with an output pulse corresponding to an absorption peak of the chromatophore. The changes in H+ binding represent a sort of “membrane Bohr effect”.

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References

  1. Schleyer, H., and Chance, B., Abst. Sixth Annual Meeting Biophys. Soc., FC9 (1962).

  2. Chance, B., Schleyer, H., and Legallais, V., in Microalgae and Photo-synthetic Bacteria (edit. by Japan Soc. Plant Physiol.), 337 (Univ. Tokyo Press, Tokyo, 1963).

    Google Scholar 

  3. Chance, B., and Schoener, B., Abst. Eighth Annual Meeting Biophys. Soc., FD9 (1964).

  4. DeVault, D., and Chance, B., Biophys. J., 6, 825 (1966).

    Article  ADS  CAS  Google Scholar 

  5. Chance, B., and Mela, L., Nature, 212, 372 (1966).

    Article  ADS  CAS  Google Scholar 

  6. Chance, B., Nishimura, M., Avron, M., and Baltscheffsky, M., Arch. Biochem. Biophys., 117, 158 (1966).

    Article  CAS  Google Scholar 

  7. Jackson, J. R., and Crofts, A. H., Europ. J. Biochem., 10, 226 (1969).

    Article  CAS  Google Scholar 

  8. Sorokin, P. P., and Lankard, J. R., IBM Res. and Dev., 10, 162 (1966).

    Article  CAS  Google Scholar 

  9. Sorokin, P. R., Lankard, J. R., Hammond, E. C., and Morruzi, V. L., IBM Res. and Dev., 11, 130 (1967).

    Article  CAS  Google Scholar 

  10. Miyazoe, Y., and Maeda, M., Appl. Phys. Lett., 12, 206 (1968).

    Article  ADS  CAS  Google Scholar 

  11. Sawyer, R. A., Experimental Spectroscopy, 240 (Dover, New York, 1963).

    Google Scholar 

  12. Chance, B., Crofts, A. R., Nishimura, M., and Price, B., Europ. J. Biochem. (in the press).

  13. Clayton, R. K., Molecular Physics in Photosynthesis (Blaisdell, New York, 1965).

    Google Scholar 

  14. Parson, W. W., Biochim. Biophys. Acta, 153, 248 (1968).

    Article  CAS  Google Scholar 

  15. Ke, B., Vernon, L. P., Garcia, A., and Ngo, E., Biochemistry, 7, 311 (1968).

    Article  CAS  Google Scholar 

  16. Ke, B., Biochim. Biophys. Acta, 172, 583 (1969).

    Article  CAS  Google Scholar 

  17. Seibert, M., and DeVault, D., Abst. Third Intern. Cong. Biophys., 1969. (in the press).

  18. Parson, W. W., Biochim. Biophys. Acta (in the press).

  19. Fleischman, D. E., and Clayton, R. K., Photochem. Photobiol., 8, 287 (1968).

    Article  CAS  Google Scholar 

  20. Bohr, C., Hasselbalch, K. A., and Krogh, A., Scand. Arch. Physiol., 16, 402 (1904).

    Article  Google Scholar 

  21. Mitchell, P., Chemiosmotic Coupling in Oxidative and Photosynthetic Phosphorylation (Glynn Research, Bodmin, 1966).

    Book  Google Scholar 

Download references

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Authors and Affiliations

  1. Johnson Research Foundation, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    B. CHANCE, J. A. McCRAY & J. BUNKENBURG

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  1. B. CHANCE
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  2. J. A. McCRAY
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  3. J. BUNKENBURG
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CHANCE, B., McCRAY, J. & BUNKENBURG, J. Fast Spectrophotometric Measurement of H+ Changes in Chromatium Chromatophores activated by a Liquid Dye Laser. Nature 225, 705–708 (1970). https://doi.org/10.1038/225705a0

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