Abstract
WHEN 17β-oestradiol and other oestrogens enter a target cell they are bound by highly specific oestrophilic proteins in a strong but non-covalent manner (Kass≃1.3 × 109–1.5 × 109 M−1 for 17β-oestradiol)1. These oestrogen receptors are found in both nuclear and cytoplasmic fractions of uterus and other target organs to this hormone1–11. We now report (1) that iodination of calf uterus oestrogen receptors in alkaline media at lowered temperature destroys their ability to bind 17β-oestradiol; (2) that this effect cannot be ascribed to oxidation of SH groups; and (3) that iodination is ineffective when the binding site is interacting with the hormone, and is thus protected from a direct effect.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Puca, G. A., and Bresciani, F., Nature, 223, 745 (1969).
Talwar, G. P., Segal, S. J., Evans, A., and Davidson, O. W., Proc. US Nat. Acad. Sci., 52, 1059 (1964).
Noteboom, W. D., and Gorski, J., Arch. Biochem. Biophys., 111, 559 (1965).
King, R. J. B., Gordon, G., and Inman, D. R., J. Endocrinology, 32, 9 (1965).
Jungblut, P. W., Hatzel, I., De Sombre, E. R., and Jensen, E. V., Colloq. Ges. Physiol. Chem., 18, 158 (Springer, Berlin, 1967).
Puca, G. A., and Bresciani, F., Nature, 218, 967 (1968).
Puca, G. A., Nola, E., and Bresciani, F., Atti Accad. Naz. Lincei (Rend. Sci. Fis. Mat. Nat.), 46, 268 (1969).
Erdos, T., Gospodarowicz, D., Bessada, R., and Fries, J., CR Acad. Sci., 266, 2164 (1968).
Korenman, S. G., and Rao, B. R., Proc. US Nat. Acad. Sci., 61, 1028 (1969).
Rochefort, H., and Baulieu, E. E., Endocrinology, 84, 108 (1969).
Brecher, P. I., and Wotiz, H. H., Endocrinology, 84, 718 (1969).
Kolthoff, I. M., and Stenger, V. A., Volumetric Analysis, 2, 273 (Interscience, New York, 1947).
Zamenhof, S., and Chargaff, E., in Methods in Enzymology (edit. by Colowick, S. P., and Kaplan, N. O.), 3, 702 (Academic Press, New York, 1957).
Strominger, J., and Lowry, O. H., J. Biol. Chem., 213, 635 (1955).
Hughes, W. L., Ann. NY Acad. Sci., 70, 3 (1957).
Jensen, E. V., Hurst, D. J., De Sombre, E. R., and Jungblut, P. W., Science, 158, 385 (1967).
Singer, S. J., in Adv. Protein Chem., 22, 1 (1967).
Covelli, I., and Wolff, J., J. Biol. Chem., 241, 4444 (1966).
Hartdegen, F. J., and Repley, J. A., Biochim. Biophys. Acta, 92, 625 (1964).
Engel, L. L., Stoffyn, A. M., and Scott, J. F., in Proc. First Intern. Cong. Hormonal Steroids, 1, 291 (Academic Press, New York, 1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PUCA, G., BRESCIANI, F. Binding Activity of Oestrogen Receptors destroyed by Iodination. Nature 225, 1251–1252 (1970). https://doi.org/10.1038/2251251a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/2251251a0
This article is cited by
-
Isolation of an estradiol-binding system from the guinea pig uterus
Bulletin of Experimental Biology and Medicine (1973)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.