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Biological Sciences: Derivation of Partial Sequence of Human β Chain Messenger RNA from Mutation Data

Nature volume 225, pages 1244–1245 (1970)Cite this article

Abstract

THREE general approaches can be used to determine the base sequence of natural messenger ribonucleic acids (mRNAs): (1) the molecular species in question can be purified and sequenced by chemical methods1; (2) purified aminoacyl-tRNAs (which are codon-specific) can be used to deliver labelled amino-acids to particular sites in a protein of known sequence2,3; and (3) the mRNA sequence can be inferred from the pattern of amino-acid substitutions in mutant proteins of known sequence4–6. It has been possible to determine indirectly the RNA sequence which codes for a carboxy-terminal segment of lysozyme4, but no intensive effort seems to have been made to apply the large amount of sequence data7 accumulated on homologous proteins to similar ends.

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Authors and Affiliations

  1. College of Physicians and Surgeons, Columbia University Institute of Cancer Research, Francis Delafield Hospital, 99 Fort Washington Avenue, New York, 10032

    KARL W. LANKS

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  1. KARL W. LANKS
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LANKS, K. Biological Sciences: Derivation of Partial Sequence of Human β Chain Messenger RNA from Mutation Data. Nature 225, 1244–1245 (1970). https://doi.org/10.1038/2251244a0

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