Abstract
THE HbO2 dissociation curves of haemolysates of the newt (Triton cristatus) have been studied at different temperatures. Blood samples were collected from single animals in 0.6 per cent NaCl solution containing EDTA as anticoagulant and centrifuged to remove the serum. The packed washed red cells were then treated with distilled water containing a drop of CC14 and haemolysates were separated in a Sorvall refrigerated centrifuge at 15,000 r.p.m.
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References
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MORPURGO, G., BATTAGLIA, P. & LEGGIO, T. Negative Bohr Effect in Newt Haemolysates and its Regulation. Nature 225, 76–77 (1970). https://doi.org/10.1038/225076a0
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DOI: https://doi.org/10.1038/225076a0
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