Abstract
DNA synthesis can be induced in all stages of the division cycle of mammalian cells by such agents as ultraviolet light1, X-irradiation2 and alkylating agents3. This so-called unscheduled DNA synthesis is of low magnitude compared with normal, semi-conservative DNA synthesis4, and has been equated to repair activity in damaged cells. We have observed that significant amounts of 3H-thymidine are incorporated into the DNA of HeLa cells without an extracellular stimulus, and at a time when normal, semi-conservative DNA synthesis does not occur. We report here the results of autoradiographic investigations of this spontaneous unscheduled DNA synthesis and its possible role in normal DNA metabolism.
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DJORDJEVIC, B., EVANS, R., PEREZ, A. et al. Spontaneous Unscheduled DNA Synthesis in G1 HeLa Cells. Nature 224, 803–804 (1969). https://doi.org/10.1038/224803a0
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DOI: https://doi.org/10.1038/224803a0
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