Abstract
THE mixed leucocyte culture test1 has been modified and adopted for the dog in our laboratory. When peripheral blood leucocytes from two unrelated people are mixed in culture for several days (the untreated cells from one individual acting as the responding population, and the mitomycin treated cells from the second individual acting as a stimulation population) a proliferative response occurs which is characterized by an increase in DNA synthesis. The same type of response seems to occur in the dog. In man and the dog, the response seems to be related to the ability of one population of cells to recognize an immunogenetic difference in the second population of cells. Lack of reactivity in this test is interpreted as meaning that two populations of cells lack immunogenetic disparity or cannot recognize the existing disparity. If the antigens of the lymphocytes are similar to those present in other organs, lack of reactivity in mixed leucocyte culture tests might lead to the prediction of long term allograft survival. This hypothesis, although incompletely tested, seems to be true of human and dog kidney transplants (unpublished results).
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Bach, F. H., and Voynow, N. K., Science, 153, 545 (1966).
Miller, J. F. A. P., Science, 144, 1544 (1964).
Miller, J. F. A. P., Doak, S. M. A., and Cross, A. M., Proc. Soc. Exp. Biol. and Med., 112, 785 (1963).
Miller, J. F. A. P., and Mitchell, G. F., Transplantation Reviews (edit. by Moller, G.), 1 (Munksgaard, in the press).
Kisken, W. A., Arch. Surg., 92, 386 (1966).
Taylor, R. B., Nature, 208, 1334 (1965); Metcalf, D., ibid., 1336; Miller, J. F. A. P., ibid., 1337.
Dumont, A. E., Human Transplantation (edit. by Rapaport, F. T., and Dausset, J. (Grune and Stratton, New York and London, 1968).
Turner, W. S., et al., Surg. Gyn. and Ob., 121, 334 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KISKEN, W., SWENSON, N. Unresponsiveness of Mixed Leucocyte Cultures from Thymectomized Adult Dogs. Nature 224, 76–77 (1969). https://doi.org/10.1038/224076a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/224076a0
This article is cited by
-
Thymus-dependent differentiation of lymphocytes in dogs assessed by tests for allogeneic lymphocytes and phytohemagglutinin
Bulletin of Experimental Biology and Medicine (1974)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.