Abstract
THE genetics of phage, bacteria and fungi owe much to the study of conditional lethal mutants, but little is known of the conditional lethal mutants of mammalian cells. I therefore wish to describe the isolation of conditional temperature sensitive mutants of African green monkey kidney cells (BS-C-1) (ref. 1). Because these cells are susceptible to simian virus (SV 40) yet less liable to transformation than other cell lines (hamster kidney cells, mouse embryo cells)2, I hope that this development will be valuable in the study of, first, control mechanism of virus–host interaction in cases where viral growth is inhibited at a restrictive temperature; second, regulatory mechanism of replication of cellular organelles; and third, cytological and morphological analyses in cultures synchronized by exposure (shock) to limiting conditions of temperature3.
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References
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NAHA, P. Temperature Sensitive Conditional Mutants of Monkey Kidney Cells. Nature 223, 1380–1381 (1969). https://doi.org/10.1038/2231380a0
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DOI: https://doi.org/10.1038/2231380a0
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