Abstract
LEUKAEMIA (lymphosarcoma) virus (FLV) has been detected in cat tissue and plasma1–3. The “C”-type virus, in addition to replicating from plasma and vacuolar membranes of a variety of cell types, is also morphologically similar to murine leukaemia viruses4. While studying in vitro bovine leukaemia our group established a successful method for propagation of lymphoid cell suspension cultures5. These lymphoid cells had a hyperdiploid chromosome complement and they were devoid of recognizable virus. Because tissue from cats with leukaemia contains “C”-type virus, we have tested to see if cells grown in suspension cultures derived from such tissue continue to replicate leukaemia virus in culture. We now report the development of a successful method for propagation of “C”-type FLV in lymphoid cells grown continuously in cell suspension, which should provide a standard and constant source of FLV.
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THEILEN, G., KAWAKAMI, T., RUSH, J. et al. Replication of Cat Leukaemia Virus in Cell suspension Cultures. Nature 222, 589–590 (1969). https://doi.org/10.1038/222589b0
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DOI: https://doi.org/10.1038/222589b0
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