Abstract
LEUKAEMIA (lymphosarcoma) virus (FLV) has been detected in cat tissue and plasma1–3. The “C”-type virus, in addition to replicating from plasma and vacuolar membranes of a variety of cell types, is also morphologically similar to murine leukaemia viruses4. While studying in vitro bovine leukaemia our group established a successful method for propagation of lymphoid cell suspension cultures5. These lymphoid cells had a hyperdiploid chromosome complement and they were devoid of recognizable virus. Because tissue from cats with leukaemia contains “C”-type virus, we have tested to see if cells grown in suspension cultures derived from such tissue continue to replicate leukaemia virus in culture. We now report the development of a successful method for propagation of “C”-type FLV in lymphoid cells grown continuously in cell suspension, which should provide a standard and constant source of FLV.
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References
Jarrett, W., Crawford, E., Martin, W., and Davie, F., Nature, 202, 568 (1964).
Rickard, C., Barr, L., Noronha, F., Dougherty, E., and Post, J., Cornell Vet., 57, 302 (1967).
Kawakami, T., Theilen, G., Dungworth, D., Munn, R., and Beall, S., Science, 158, 1049 (1967).
Theilen, G., Kawakami, T., Dungworth, D., Switzer, J., Munn, R., and Harrold, J., J. Amer. Vet. Med. Assoc., 153, 1864 (1968).
Theilen, G., Rush, J., Nelson-Rees, W., Dungworth, D., Munn, R., and Switzer, J., J. Nat. Cancer Inst., 40, 737 (1968).
Leibovitz, A., Amer. J. Hyg., 78, 173 (1963).
Clarkson, B., Strife, A., and DeHarven, E., Cancer, 20, 926 (1967).
Jarrett, O., Laird, H., Hoy, D., and Crighton, G., Nature, 219, 521 (1968).
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THEILEN, G., KAWAKAMI, T., RUSH, J. et al. Replication of Cat Leukaemia Virus in Cell suspension Cultures. Nature 222, 589–590 (1969). https://doi.org/10.1038/222589b0
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DOI: https://doi.org/10.1038/222589b0
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