Abstract
A HIGHLY cross-linked polyacrylamide gel containing a trapped protein antigen has been prepared. It can be used as an immunosorbent. The principle of the method is based on those of Bernfeld and Wan1 and Hicks and Ubdike2 for dissolving enzymes for chemical analysis. Their protein-polyacrylamide gel is, however, of no practical use as an immunosorbent, for its permeability is too low to enable antibodies in solution to enter the gel and react with the trapped antigens. Fawcett and Morris3 have shown that by changing the total monomer concentration a marked increase in gel permeability can be achieved.
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References
Bernfeld, P., and Wan, J., Science, 142, 678 (1963).
Hicks, G. P., and Ubdike, S. J., Anal. Chem., 38, 726 (1966).
Fawcett, J. S., and Morris, C. J. O. R., Separation Sci., 1, 9 (1966).
Hjerten, S., Arch. Biochem. Biophys., Suppl., 1, 147 (1962).
Avrameas, S., and Ternynck, R., Biochem. J., 102, 370 (1967).
Kabat, E. A., and Meyer, M. M., Experimental Immunochemistry, chap. 2, Fig. 4 (Charles C. Thomas, Springfield, Illinois, 1964).
Gyenes, L., Rose, E., and Sehon, A. H., Nature, 181, 1465 (1958).
Axen, R., Porath, J., and Ernback, S., Nature, 214, 1302 (1967).
Centeno, E. R., and Sehon, A. H., Fed. Proc., 25, 729 (1966).
Onoue, K., Yagi, Y., and Pressman, D., Immunochemistry, 2, 181 (1965).
Avrameas, S., and Ternynck, R., J. Biol. Chem., 242, 1651 (1967).
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CARREL, S., GERBER, H. & BARANDUN, S. Preparation of Polyacrylamide Gels which contain Protein and their Use as High Capacity Immunosorbents. Nature 221, 385–386 (1969). https://doi.org/10.1038/221385a0
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DOI: https://doi.org/10.1038/221385a0
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