Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Infectivity of Bacteriophage R17 RNA after Sequential Removal of 3′ Terminal Nucleotides

Nature volume 221, pages 321–325 (1969)Cite this article

Abstract

The 3′ terminal adenylate of R17 RNA can be removed without destroying the infectivity of the RNA. The penultimate cytidylate, however, is required. Progeny molecules from spheroplasts infected with RNA missing the terminal A have the normal 3′ end restored.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Weissman, C., Borst, P., Burdon, R. H., Billeter, M. A., and Ochoa, S., Proc. US Nat. Acad. Sci., 51, 682 (1964).

    Article  ADS  Google Scholar 

  2. Erickson, R. L., Fenwick, M. L., and Franklin, R. M., J. Mol. Biol., 10, 519 (1964).

    Article  Google Scholar 

  3. Ammann, J., Delius, H., and Hofschneider, P. H., J. Mol. Biol., 10, 557 (1964).

    Article  CAS  PubMed  Google Scholar 

  4. Billeter, M. A., Libonati, M., Vinuela, E., and Weissman, C., J. Mol. Biol., 241, 4750 (1966).

    CAS  Google Scholar 

  5. Weissman, C., and Feix, G., Proc. US Nat. Acad. Sci., 55, 1264 (1966).

    Article  ADS  Google Scholar 

  6. Mills, D. R., Pace, N. R., and Spiegelman, S., Proc. US Nat. Acad. Sci., 56, 1778 (1966).

    Article  ADS  CAS  Google Scholar 

  7. Pace, N. R., Bishop, D. H. C., and Spiegelman, S., Proc. US Nat. Acad. Sci., 58, 711 (1967).

    Article  ADS  CAS  Google Scholar 

  8. Feix, G., Pollel, R., and Weissman, C., Proc. US Nat. Acad. Sci., 59, 145 (1968).

    Article  ADS  CAS  Google Scholar 

  9. Weith, H. L., Asteriadis, G. T., and Gilham, P. T., Science, 160, 1459 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  10. Dahlberg, J., Nature, 220, 548 (1968).

    Article  ADS  CAS  PubMed  Google Scholar 

  11. Bishop, D. H. L., Pace, N. R., and Spiegelman, S., Proc. US Nat. Acad. Sci., 58, 1790 (1967).

    Article  ADS  CAS  Google Scholar 

  12. Steinschneider, A., and Fraenkel-Conrat, H., Biochemistry, 5, 2735 (1966).

    Article  CAS  PubMed  Google Scholar 

  13. deWachter, R., and Fiers, W., J. Mol. Biol., 30, 507 (1967).

    Article  CAS  Google Scholar 

  14. Weith, H. L., and Gilham, P. T., J. Amer. Chem. Soc., 89, 5473 (1967).

    Article  CAS  Google Scholar 

  15. Hecht, L. J., Zamencnik, P. C., Stephenson, M. L., and Scott, J. F., J. Biol. Chem., 233, 954 (1958).

    CAS  PubMed  Google Scholar 

  16. Preiss, J., Diechmann, M., and Berg, P., J. Biol. Chem., 236, 1748 (1961).

    CAS  PubMed  Google Scholar 

  17. Furth, J. J., Hurwitz, J., Krug, R., and Alexander, M., J. Biol. Chem., 236, 3317 (1961).

    CAS  PubMed  Google Scholar 

  18. Daniel, V., and Littauer, U. Z., J. Biol. Chem., 238, 2102 (1963).

    CAS  PubMed  Google Scholar 

  19. Whitfeld, P. R., and Markham, R., Nature, 171, 1151 (1953).

    Article  ADS  CAS  PubMed  Google Scholar 

  20. Whitfeld, P. R., Biochem. J., 58, 390 (1954).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  21. Bishop, D. H. L., Claybrook, J. R., and Spiegelman, S., J. Mol. Biol., 26, 373 (1967).

    Article  CAS  PubMed  Google Scholar 

  22. Gesteland, R. F., and Boedtker, H., J. Mol. Biol., 8, 496 (1964).

    Article  CAS  PubMed  Google Scholar 

  23. Leppla, S. M., Bjoraker, B., and Bock, R. M., Methods in Enzymology, XIIB, 236 (1965).

    Google Scholar 

  24. Pace, N. R., and Spiegelman, S., Proc. US Nat. Acad. Sci., 55, 1608 (1966).

    Article  ADS  CAS  Google Scholar 

  25. Scholtissek, C., Biochim. Biophys. Acta, 61, 499 (1962).

    CAS  Google Scholar 

  26. Lacks, S., and Gros, F., J. Mol. Biol., 1, 39 (1959).

    Article  Google Scholar 

  27. Cannon, M., Biochim. Biophys. Acta, 87, 154 (1964).

    CAS  PubMed  Google Scholar 

  28. Rosset, R., and Monier, R., Biochim. Biophys. Acta, 108, 376 (1965).

    Article  CAS  PubMed  Google Scholar 

  29. Rosset, R., and Monier, R., Biochim. Biophys. Acta, 108, 385, (1965).

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biological Laboratories, Harvard University, Cambridge, Massachusetts

    ROBERT KAMEN

Authors
  1. ROBERT KAMEN
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

KAMEN, R. Infectivity of Bacteriophage R17 RNA after Sequential Removal of 3′ Terminal Nucleotides. Nature 221, 321–325 (1969). https://doi.org/10.1038/221321a0

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1038/221321a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing