Abstract
ACTINOMYCIN D (AM) suppresses DNA-directed RNA synthesis in vivo1. Because the nucleolus is believed to be the site of ribosomal RNA synthesis2,3, the effect of AM on this organelle is of particular interest and has been the subject of many studies4–7. It has been reported that both the morphological and physiological effects of AM can, in certain circumstances, be reversed8,9. In view of the extensive use of AM in cancer chemotherapy as well as in cellular metabolic studies, it is important to establish whether cell constituents such as the nucleolus can recover their normal function after temporary exposure to concentrations of AM known to be lethal to the intact cell. This question can only be answered by transplanting the nucleoli treated with drugs to normal cytoplasm—a procedure most easily accomplished in the large, free-living amoebae by nuclear transplantation. The object of this work is to determine to what extent the effect of lethal concentrations of AM on the amoeba nucleoli can be reversed.
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LORCH, I., JEON, K. Reversible Effect of Actinomycin D on Nucleoli of Amoeba discoides : Nuclear Transplantation Study. Nature 221, 1073–1074 (1969). https://doi.org/10.1038/2211073a0
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DOI: https://doi.org/10.1038/2211073a0
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