Abstract
THERE have been several reports of the in vivo effects of phytohaemagglutinin (PHA) on the primary immune response, but the conclusions are equivocal and the mode of action of PHA is still far from clear. Elves1 has recently shown that PHA acts as an immunosuppressor exclusively on the production of humoral antibody, provided both the PHA and the antigen are given intraperitoneally. Apart from this special case2–6, the effect of PHA on immunological reactions has been stimulatory rather than suppressive7–10. Histological examinations11–13 and diffusion chamber experiments3 have shown that in vivo treatment with PHA results in a pronounced increase in the number of immunoblast-like cells. Nevertheless it is not yet clear whether this effect has any relationship to the immunological results. We therefore decided to determine whether PHA has an effect on the response of mice to heterologous erythrocytes, as judged by their ability to produce the plaque forming cells using the Jerne technique, and to investigate the morphology of the antibody producing cells observed.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Elves, M. W., Intern. Arch. Allergy, 37, 353 (1968).
Elves, M. W., Nature, 213, 494 (1967).
Gengozian, N., and Hübner, K. F., J. Immunol., 99, 184 (1967).
Spreafico, F., and Lerner, E. M., J. Immunol., 98, 407 (1967).
Jennings, J. F., and Oates, G. M., Clin. Exp. Immunol., 2, 445 (1967).
Markley, K., Smallman, E. and Evans, G., Intern. Arch. Allergy, 32, 482 (1967).
Gamble, C. N., Intern. Arch. Allergy, 29, 470 (1966).
Singhal, S. K., Naspitz, C. K., and Richter, M., Intern. Arch. Allergy, 31, 390 (1967).
Elves, M. W., Transplantation, 5, 1532 (1967).
Gilette, R., Goulain, D., and Conway, H., First Intern. Cong. of Transplant. Soc. Paris, Abstr. 147 (1967).
Gamble, C. N., Blood, 28, 175 (1966).
Naspitz, C. K., Blennerhassett, J. B., Singhal, S. K., and Richter, M., Intern. Arch. Allergy, 33, 389 (1968).
Epstein, L. B., and Smith, C. W., J. Immunol., 100, 421 (1968).
Sterzl, J., and Riha, T., Nature, 208, 859 (1965).
Takátsy, G., Acta Microbiol. Acad. Sci. Hung., 4, 365 (1955).
Uhr, J. W., and Finkelstein, M. S., J. Exp. Med., 117, 457 (1963).
Jánossy, Gy., Petrányi, jun., Gy., and Alföldy, P., Fol. Biol. (in the press).
Ling, N. P., and Holt, L. P. J., J. Cell. Sci., 2, 57 (1967).
Greaves, M. F., Roitt, I. M., Zamir, R., and Carnarghan, R. B. A., Lancet, ii, 1317 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
PETRANYI, G., JÁNOSSY, G. & ALFÖLDY, P. Effect of Phytohaemagglutinin on Plaque Forming Cells in the Mouse Spleen. Nature 221, 76–78 (1969). https://doi.org/10.1038/221076a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/221076a0
This article is cited by
-
The effect of E-N-l-trimethyllysine (TML) on the humoral and cellular immune response
Agents and Actions (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.