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Amber Suppression: a Nucleotide Change in the Anticodon of a Tyrosine Transfer RNA

Nature volume 217, pages 1019–1024 (1968)Cite this article

Abstract

In certain mutants a single base change alters the meaning of a messenger codon in such a way that, instead of spelling out an amino-acid, it spells out chain termination. Mutants in a quite different gene, called a suppression gene, allow the chain-terminating triplet to be read as an amino-acid. Experiments have shown that this is caused by a mutated tRNA which carries a single base change in its anticodon. This allows it to read the chain-terminating codon as if it spelt tyrosine.

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References

  1. Weigert, M. G., Galluci, E., Lanka, E., and Garen, A., Cold Spring Harbor Symp. Quant. Biol., 31, 145 (1966); Stretton, A. O. W., Kaplan, S., and Brenner, S., ibid., 31, 173 (1966).

    Article  CAS  Google Scholar 

  2. Capecchi, M., and Gussin, G., Science, 149, 417 (1965).

    Article  ADS  CAS  Google Scholar 

  3. Engelhardt, D. L., Webster, R. E., Wilhelm, R. C., and Zinder, N. D., Proc. US Nat. Acad. Sci., 54, 1791 (1965).

    Article  ADS  CAS  Google Scholar 

  4. Smith, J. D., Abelson, J. N., Clark, B. F. C., Goodman, H. M., and Brenner, S., Cold Spring Harbor Symp. Quant. Biol., 31, 479 (1966).

    Article  CAS  Google Scholar 

  5. Gesteland, R. F., Salser, W., and Bolle, A., Proc. US Nat. Acad. Sci., 58, 2036 (1967).

    Article  ADS  CAS  Google Scholar 

  6. Landy, A., Abelson, J., Goodman, H. M., and Smith, J. D., J. Mol. Biol., 29, 457 (1967).

    Article  CAS  Google Scholar 

  7. von Portatius, H., Doty, P., and Stephenson, M. L., J. Amer. Chem. Soc., 83, 3351 (1961).

    Article  Google Scholar 

  8. Katchalsky, E., Yankofsky, S., Novogradsky, A., Galenter, Y., and Littauer, U. Z., Biochim. Biophys. Acta, 123, 641 (1966).

    Article  Google Scholar 

  9. Sanger, F., Brownlee, G. G., and Barrell, B. G., J. Mol. Biol., 13, 373 (1965).

    Article  CAS  Google Scholar 

  10. Brownlee, G. G., and Sanger, F., J. Mol. Biol., 23, 337 (1967).

    Article  CAS  Google Scholar 

  11. Brownlee, G. G., Sanger, F., and Barrell, B. G., J. Mol. Biol. (in the press).

  12. Madison, J. T., Everett, G. A., and Kung, H. K., Science, 153, 531 (1966).

    Article  ADS  CAS  Google Scholar 

  13. Crick, F. H. C., J. Mol. Biol., 19, 548 (1966).

    Article  CAS  Google Scholar 

  14. Doctor, B. P., Loebel, J. E., and Kellogg, D. A., Cold Spring Harbor Symp. Quant. Biol., 31, 543 (1966).

    Article  CAS  Google Scholar 

  15. Appleyard, R. K., Genetics, 39, 440 (1954).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Nishimura, S., Harada, F., Narushima, U., and Seno, T., Biochim. Biophys. Acta, 142, 133 (1967).

    Article  CAS  Google Scholar 

  17. Kelmers, A. D., Novelli, G. D., and Stulberg, M. P., J. Biol. Chem., 240, 3979 (1965).

    CAS  PubMed  Google Scholar 

  18. Gillam, I., Millward, S., Blew, D., von Tigerstrom, M., Wimmer, E., and Tener, G. M., Biochemistry, 6, 3043 (1967).

    Article  CAS  Google Scholar 

  19. Holley, R. W., Apgar, J., Everett, G. A., Madison, J. T., Marquisee, M., Merrill, S. H., Penswick, J. R., and Zamir, A., Science, 147, 1462 (1965).

    Article  ADS  CAS  Google Scholar 

  20. Zachau, H. G., Dutting, D., and Feldman, H., Ang. Chemie, 5, 122 (1966).

    Google Scholar 

  21. Bayer, A. A., Venkstern, T. V., Mirsabekov, A. D., Krutilina, A. I., Li, L., and Axelrod, V. D., Molecular Biology (USSR), 1, 754 (1967).

    Google Scholar 

  22. Raj Bhandary, U. L., Chang, S. H., Stuart, A., Faulkner, R. D., Hoskinson, R. M., and Khorana, H. G., Proc. US Nat. Acad. Sci., 57, 751 (1967).

    Article  ADS  CAS  Google Scholar 

  23. Doctor, B. P., and Mudd, J., J. Biol. Chem., 238, 3677 (1963).

    CAS  PubMed  Google Scholar 

  24. Signer, E. R., Beckwith, J. R., and Brenner, S., J. Mol. Biol., 14, 153 (1965).

    Article  CAS  Google Scholar 

  25. Weigert, M. G., Lanka, E., and Garen, A., J. Mol. Biol., 23, 401 (1967).

    Article  CAS  Google Scholar 

  26. Garen, A., Garen, S., and Wilhelm, R. C., J. Mol. Biol., 14, 167 (1965).

    Article  CAS  Google Scholar 

  27. Nirenberg, M., and Leder, P., Science, 145, 1399 (1964).

    Article  ADS  CAS  Google Scholar 

  28. Lodish, H. F., Abelson, J., and Clark, B. F. C., Abst. of Fed. Europ. Biochem. Soc., Fourth Meeting, Oslo (1967).

    Google Scholar 

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Authors and Affiliations

  1. MRC Laboratory of Molecular Biology, Cambridge

    HOWARD M. GOODMAN, JOHN ABELSON, ARTHUR LANDY, S. BRENNER & J. D. SMITH

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GOODMAN, H., ABELSON, J., LANDY, A. et al. Amber Suppression: a Nucleotide Change in the Anticodon of a Tyrosine Transfer RNA. Nature 217, 1019–1024 (1968). https://doi.org/10.1038/2171019a0

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