Abstract
NORMAL human glucose-6-phosphate dehydrogenase (D-glucose 6-phosphate: nicotinamide adenine dinucleotide phosphate oxidoreductase, EC 1.1.1.49) (G-6-PD) can be dissociated into smaller sub-units under certain conditions1–4, which suggests that normal and variant human G-6-PD may be hybridized in vitro. Studies of such hybridization may provide information on the polypeptide chains in which mutation occurred. Hybridization between human and rat G-6-PD has been observed5, but despite the attempts made6, no successful hybridization has so far been reported between the normal and variant human G-6-PD. We report here in vitro hybridization of the normal (B +), the common Negro variant with normal red cell enzyme activity (A +) and the Seattle variant7.
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YOSHIDA, A., STEINMANN, L. & HARBERT, P. In vitro Hybridization of Normal and Variant Human Glucose-6-phosphate Dehydrogenase. Nature 216, 275–276 (1967). https://doi.org/10.1038/216275a0
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DOI: https://doi.org/10.1038/216275a0
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