Abstract
FOLLICLE stimulating hormone (FSH) was obtained from powdered human pituitary dried with acetone and purified in two ways: (a) by the method of Amir, Barker, Butt and Crooke1, and (b) by the cellosolve extraction procedure of Butt, Crooke and Wolf2 followed by fractionation on DEAE-‘Sephadex A-50’. Haemagglutination inhibition tests showed that luteinizing hormone (LH) and albumin were present to an extent of less than 1 per cent by weight of protein. FSH obtained by both procedures seems to undergo reversible association and dissociation, as the following experiments show.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Amir, S. M., Barker, S. A., Butt, W. R., and Crooke, A. C., Nature, 209, 1092 (1966).
Butt, W. R., Crooke, A. C., and Wolf, A., in Gonadotropins: Physicochemical and Immunological Properties, Ciba Foundation Study Group, No. 22 (edit. by Wolstenholme, G. E. W.), 85 (J. and A. Churchill, Ltd., London, 1965).
Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961).
Odell, W. D., Swain, R. W., and Nydick, M., J. Clin. Endocrinol. Metab., 24 (12), 1266 (1964).
Li, C. H., and Starman, B., Nature, 202, 291 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GRAY, C. Molecular Weight of Human Follicle Stimulating Hormone. Nature 216, 1112–1113 (1967). https://doi.org/10.1038/2161112a0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1038/2161112a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.