Abstract
Kent and Mallard1 and Truby and Goldzieher2 have recently indicated that free radicals formed in organic omaterial are artefacts, apparently resulting from lyophilization. Our findings are not in agreement with this concept. We believe that lyophilization of living biological materials, of and by itself, produces few if any free radicals. Our investigations indicate that the electron paramagnetic resonance signals observed by others3–5 were the result of a reaction between oxygen and an unidentified component in dried specimens which formed free radicals during the interval between lyophilization and observation in the electron paramagnetic resonance spectrometer.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kent, M., and Mallard, J. R., Nature, 215, 736 (1967).
Truby, F. K., and Goldzieher, J. W., Nature, 182, 1371 (1958).
Dettmer, D. M., Driscoll, D. H., and Wallace, J. D., Nature, 214, 492 (1967).
Morozova, G. K., and Bluymenfel'd, L. A., Biophysics, 5, 273 (1960).
Commoner, B., Townsend, J., and Pake, G. E., Nature, 174, 689 (1954).
Dimmick, R. L., Heckly, R. J., and Hollis, D. P., Nature, 192, 776 (1961).
Heckly, R. J., Dimmick, R. L., and Windle, J. J., J. Bacteriol., 85, 961 (1963).
Heckly, R. J., Cryobiology, 2, 139 (1965).
Miyagawa, I., Gordy, W., Watabe, N., and Wilbur, K. M., Proc. US Nat. Acad. Sci., 44, 613 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HECKLY, R., DIMMICK, R. Electron Paramagnetic Resonance in Frozen and Dried Biological Materials. Nature 216, 1003–1004 (1967). https://doi.org/10.1038/2161003a0
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1038/2161003a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.