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Polyacrylamide Gel-electrophoresis across a Molecular Sieve Gradient

Nature volume 214, pages 1334–1336 (1967)Cite this article

Abstract

THE “molecular sieve” effect1–5 of polyacrylamide gel is closely related to the concentration of the gel2, which may be varied over wide limits. The estimates of the “average pore size” differ somewhat, but appear to be roughly in the region of 20, 50 and 150 Å at polyacrylamide concentrations of 20, 7.5 and 3 per cent, respectively2,5, although it is still not clear how much this is affected by the degree of crosslinkage6.

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Authors and Affiliations

  1. Children's Medical Research Foundation, Royal Alexandra Hospital for Children, Camperdown, Sydney, Australia

    J. MARGOLIS & K. G. KENRICK

Authors
  1. J. MARGOLIS
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  2. K. G. KENRICK
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MARGOLIS, J., KENRICK, K. Polyacrylamide Gel-electrophoresis across a Molecular Sieve Gradient. Nature 214, 1334–1336 (1967). https://doi.org/10.1038/2141334a0

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