Abstract
A MAJOR problem associated with the in vitro cultivation of specialized tissues is their failure to maintain their specific functions. Even in some situations where the specialized cells have been shown to survive, they begin to lose their unique functions1. The secretory cell of the mammary gland is an outstanding example of a cell where this dedifferentiation may be readily determined by following in culture the multitude of functions unique to the synthesis of milk. These decrease and eventually disappear but at different rates2–5. Changes in other functions have also been observed. The specific activity of lactate dehydrogenase (LDH) decreases in culture for a week or two and then increases and levels off with time3. This change in the activity of LDH raised the question as to whether it was merely quantitative or if it could be associated with a change in the cellular isozymic complement.
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BLANCO, A., RIFÉ, U. & LARSON, B. Lactate Dehydrogenase Isozymes during Dedifferentiation in Cultures of Mammary Secretory Cells. Nature 214, 1331–1333 (1967). https://doi.org/10.1038/2141331b0
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DOI: https://doi.org/10.1038/2141331b0
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