Abstract
DURING an investigation of the opalescence produced in serum by some strains of Streptococcus pyogenes1 it was noticed that the streptococcal cell wall could be separated into two roughly equal fractions by the action of 1 per cent sodium deoxycholate solution. Most of the methods used in cell wall fractionation involve extreme acid pH. (ref. 2) or high temperatures3 or both4, so that the use of sodium deoxycholate at 0° C would present an interesting alternative to the conventional procedures. The general applicability of the deoxycholate extraction method was therefore investigated.
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HILL, M. Action of Bile Salts on Bacterial Cell Walls. Nature 214, 1152–1154 (1967). https://doi.org/10.1038/2141152a0
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DOI: https://doi.org/10.1038/2141152a0
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