Abstract
THERE have been a number of studies on the effects of ultra-violet microbeam irradiation of delimited areas within cells in culture. In experiments with mitotic cells1–10, it has been found that irradiation of a chromosome produces a loss of DNA and histone at the site of irradiation3,4,9, and also that the spindle diminishes in size or disappears temporarily following the irradiation of any part of the cell1,2,5–8,10. In experiments with interphase cells11,12, some authors have reported that about two-thirds of the labelling of cytoplasmic RNA is suppressed by nucleolar irradiation11, while extranucleolar nuclear irradiation results in a progressive inhibition of DNA synthesis for several hours after the irradiation12. We have carried out an autoradiographic study on the effects of ultra-violet microbeam irradiation of different cellular sites on synthesis of RNA, DNA and protein in order to compare the pattern of inhibition in a single cell type.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Uretz, R. B., and Zirkle, R. E., Biol. Bull., 109, 370 (1955).
Zirkle, R. E., Uretz, R. B., and Hayness, R. H., Ann. N.Y. Acad. Sci., 90, 435 (1960).
Bloom, W., and Leider, R. J., J. Cell Biol., 13, 269 (1962).
Bloom, W., and Ozarslan, S., Proc. U.S. Nat. Acad. Sci., 53, 1294 (1965).
Izutsu, K., Mie Med. J., 9, 15 (1959).
Izutsu, K., Mie Med. J., 11, 199 (1961).
Izutsu, K., Mie Med. J., 11, 213 (1961).
Takeda, S., and Izutsu, K., Jap. J. Genet., 36 Suppl. 105 (1961).
Takeda, S., Symposia Cell Chem., 14, 35 (1964).
Yashima, Y., Mie Med. J., 12, 215 (1962).
Perry, R. P., Hell, A., and Errera, M., Biochim. Biophys. Acta, 49, 47 (1961).
Dendy, P. P., and Smith, C. L., Proc. Roy. Soc., B, 160, 328 (1964).
Ficq, A., The Cell, 1, 67 (Academic Press, New York and London, 1959).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
TAKEDA, S., NARUSE, S. & YATANI, R. Effects of Ultra-violet Microbeam Irradiation of Various Sites in HeLa Cells on the Synthesis of RNA, DNA and Protein. Nature 213, 696–697 (1967). https://doi.org/10.1038/213696a0
Issue Date:
DOI: https://doi.org/10.1038/213696a0
This article is cited by
-
�ber die wirkung von ultraviolettem licht auf menschliche chromosomen
Archives for Dermatological Research (1975)
-
Effect of Ultraviolet Light on RNA and Protein Synthesis in Differentiated Epidermal Cells
Nature (1967)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
