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Effect of Acetylation on the Biological Activity of Pregnant Mare Serum Gonadotrophin

Nature volume 213, pages 1029–1030 (1967)Cite this article

Abstract

DURING our recent investigation into the mechanism of action of pregnant mare serum gonadotrophin (PMS)1, we prepared a sample of highly radioactive peptide hormone. Radioactive steroid hormones are relatively easy to prepare and have been utilized in investigations on the mechanism of action of these hormones2. More recently, radioactive peptide hormones have been reported3–5 to have similar biological and antigenic properties to the native proteins. Acetylation of bovine growth hormone, utilizing tritiated acetic anhydride, resulted in a sample of hormone biologically and antigenically equivalent to the native hormone3, while treatment of ACTH under similar conditions led to a loss of hormonal activity3. The results of our attempts to acetylate PMS are now reported.

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References

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Author notes

  1. G. C. BECKING

    Present address: Food and Drug Research Laboratories, Ottawa, Canada

Authors and Affiliations

  1. Chemistry and Biomedical Departments, Pitman-Moore Division, The Dow Chemical Company, Indianapolis, Indiana

    G. C. BECKING & J. F. HAYDEN

Authors
  1. G. C. BECKING
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  2. J. F. HAYDEN
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BECKING, G., HAYDEN, J. Effect of Acetylation on the Biological Activity of Pregnant Mare Serum Gonadotrophin. Nature 213, 1029–1030 (1967). https://doi.org/10.1038/2131029a0

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  • DOI: https://doi.org/10.1038/2131029a0

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