Abstract
EARL1 reported the maintenance of microfilariae in mixture 199 plus dog serum for 61 days. Sawyer and Weinstein2 and Weinstein3 reported development of the microfilariae of Dirofilaria immitis and Wuchereria bancrofti, respectively, to late first-stage (“sausage-form”) larvae. This communication describes the in vitro development of Macacanema formosana microfilariae through second- and possibly to third-stage larvae in the presence of Aedes aegypti cells in culture.
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References
Earl, P. R., Ann. N. Y. Acad. Sci., 77, 163 (1959).
Sawyer, T. K., and Weinstein, P. P., J. Parasitol., 49, 218 (1963).
Weinstein, P. P., Trans. Roy. Soc. Trop. Med. Hyg., 57, 236 (1963).
Grace, T. D. C., Nature, 211, 367 (1966).
Suitor, jun., E. C. (unpublished work).
Grace, T. D. C., J. Gen. Physiol., 41, 1027 (1958).
Grace, T. D. C., Nature, 195, 788 (1962).
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WOOD, D., SUITOR, E. In vitro Development of Microfilariae of Macacanema formosana in Mosquito Cell Cultures. Nature 211, 868–870 (1966). https://doi.org/10.1038/211868b0
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DOI: https://doi.org/10.1038/211868b0
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