Abstract
WE have recently conducted an investigation of the factors involved in the release or retention of lactate dehydrogenase (LDH) from the particulate fraction of chicken breast muscle1. By appropriate procedures, we could effect essentially a complete release or retention. The major factors were the pH and ionic strength of the homogenization media; high pH and high ionic strength caused a greater solubilization of the enzyme. The influence of other factors could be explained in terms of their effect on pH. and ionic strength. Adjusting conditions for the retention of LDH activity in the particulate fraction does not, however, establish that this enzyme occurs in a particulate or bound form in situ. Other possibilities such as co-precipitation, adsorption, and physical entrapment must be considered.
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HULTIN, H., WESTORT, C. & SOUTHARD, J. Adsorption of Lactate Dehydrogenase to the Particulate Fraction of Homogenized Skeletal Muscle. Nature 211, 853–854 (1966). https://doi.org/10.1038/211853a0
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DOI: https://doi.org/10.1038/211853a0
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