Abstract
THE procedure described by Hunter and Greenwood1 with some minor modifications has been used in this laboratory for small-scale preparation of iodine-131-labelled insulin. About 1.2 mc. iodine-131 from Kjeller is used in a volume of less than 0.025 ml. for the labelling of 5 µg of purified insulin. The iodination efficiency is usually in the region of 60–80 per cent, giving a specific activity of about 150 µc. iodine-131 per µg insulin.
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Hunter, W. M., and Greenwood, F. C., Nature, 194, 495 (1962).
Manipol, V., and Spitzy, H., Int. J. Appl. Rad. Is., 13, 647 (1962).
Banerjee, R. N., and Gibson, K., J. Endocrin., 25, 145 (1962).
Merimee, T. J., and Prout, T. E., Metabolism, 14, No. 2, 115 (1965).
Robinson, F. A., and Fehr, K. L. A., Biochem. J., 51, 298 (1952).
Grassini, G., and Lederer, M., J. Chrom., 2, 326 (1959).
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HØYE, Å. Purification of Insulin labelled with Iodine-131. Nature 211, 746 (1966). https://doi.org/10.1038/211746a0
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DOI: https://doi.org/10.1038/211746a0
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