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Biological Detection of Phytochrome in Callus Tissue of Nicotiana tabacum L.

Abstract

DURING recent years, considerable information has been gained concerning the role of light in germination, etiolation, and control of flowering. The discovery of phytochrome has greatly aided the understanding of these processes. Briggs and Siegelman1 found that seedling plants had highest phytochrome concentrations in regions of active cell division. We wished to know whether or not phytochrome had any function in actively dividing cells cultured from undifferentiated stem pith. This communication reports evidence that the phytochrome system was involved in growth of callus tissue cultured in vitro from undifferentiated pith from stems of Nicotiana tabacum L.

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References

  1. Briggs, W. R., and Siegelman, H. W., Plant Physiol., Suppl., 38, v (1963).

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  2. Murashige, T., and Skoog, F., Physiol. Plantarum, 15, 473 (1962).

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  3. Kasperbauer, M. J., Borthwick, H. A., and Hendricks, S. B., Bot. Gaz., 124, 444 (1963).

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KASPERBAUER, M., REINERT, R. Biological Detection of Phytochrome in Callus Tissue of Nicotiana tabacum L.. Nature 211, 744–745 (1966). https://doi.org/10.1038/211744a0

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