Abstract
THE radiohomogeneity of tritium- and carbon-14-labelled methyl linoleate in vivo and the stability of the labelled methyl esters during gas–liquid chromatography has recently been investigated1. It was shown that the tritium : carbon-14 ratio of the entire peak was unchanged by gas–liquid chromatography. However, when portions of the effluent peak were collected the tritium : carbon-14 ratio varied. In view of the separation potentialities of gas chromatography, it seemed worthwhile to investigate whether or not any difference in the mobilities of the two isotopic molecules during chromatography caused these variations. Accordingly, several columns containing commonly used polar and non-polar phases were used and a number of methyl esters labelled with both tritium and carbon-14 were chromatographed. The tritium : carbon-14 ratios obtained for successive fractions were examined for evidence of any difference in the mobilities of the two compounds.
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SGOUTAS, D. Isotope Fractionation of Methyl Esters by Gas–Liquid Chromatography. Nature 211, 296–297 (1966). https://doi.org/10.1038/211296a0
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DOI: https://doi.org/10.1038/211296a0
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