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Purification and Characterization of Ribonuclease from Unfertilized Eggs of the Sea Urchin, Psammechinus miliaris

Abstract

KNOWLEDGE about the various types of RNA involved in the biosynthesis of proteins has increased greatly over the past few years. Little is known, however, about the part played by the various ribonucleases (RNases) known to be present in most cells, in the metabolism of the RNA. There has been much speculation on this topic, but few attempts have been made to investigate the physiological action of these substances. This is largely because few of the many RNases identified have been isolated and purified sufficiently to allow them to be characterized. The purification of RNase has great interest at present because of its possible use in the structural analysis of RNA molecules.

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FERNLUND, P., JOSEFSSON, L. Purification and Characterization of Ribonuclease from Unfertilized Eggs of the Sea Urchin, Psammechinus miliaris. Nature 211, 1402–1403 (1966). https://doi.org/10.1038/2111402a0

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