Abstract
So far there has been no really satisfactory method for the quantitative separation in high-molecular form of a mixture of DNA and RNA isolated from cells. The procedure based on the different solubility of DNA and RNA in salt solutions of varying concentration1–3 is quantitatively unsatisfactory. Another method uses chromatographic separation on methylated albumin4–7, but involves a series of steps which entail the danger of degradation in the recovery of the macromolecule. Specifically, single-stranded DNA cannot be quantitatively recovered without loss of its high-molecular character, because complete elution occurs only at strongly alkaline pH. Nygaard and Hall8 have recently described a micro method for the detection of DNA/RNA hybrids based on their adsorption on membrane niters of nitrocellulose containing material.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Aldridge, W. G., Nature, 187, 323 (1960); 192, 284 (1962). See below (ref. 10).
Colter, J. S., Brown, R. A., and Ellem, K. A. O., Biochim. Biophys. Acta, 55, 31 (1962).
Jones, A. S., Nature, 199, 280 (1963).
Mandell, J. D., and Hershey, A. D., Anal. Biochem., 1, 66 (1960).
Hershey, A. D., and Burgi, E., J. Mol. Biol., 2, 143 (1960).
Suoeka, N., and Cheng, T. Y., J. Mol. Biol., 4, 161 (1962).
Ishihama, A., Mizuno, N., Takai, M., Otaka, E., and Osawa, S., J. Mol. Biol., 5, 251 (1962).
Nygaard, A. P., and Hall, B. D., Biochem. Biophys. Res. Comm., 12, 98 (1963).
Kirby, K. S., Biochem. J., 64, 405 (1956).
Burton, K., Biochem. J., 62, 315 (1956).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KLAMERTH, O. Separation of High-molecular Deoxyribonucleic Acid and Ribonucleic Acid. Nature 208, 1318–1319 (1965). https://doi.org/10.1038/2081318a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/2081318a0
This article is cited by
-
DNA Circularization: a Molecular Approach for the Detection and Isolation of Tandemly Duplicated Genes
Nature New Biology (1971)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.