Abstract
GIANT polytene chromosomes from the salivary glands of various fly larvae have been extensively examined; because of their enormous size they have become one of the most important tools in investigations of gene activity and nuclear-cytoplasmic relationships. Malpighian tubules and certain other tissues of flies also possess polytene chromosomes, but they show a lower degree of polyteny. It is generally accepted that in most animal and plant cells there is a constant relation between the DNA content and the number of chromosome ‘sets’ it contains. Synthesis of DNA results in a doubling of this pre-existing amount; a dipteran polytene chromosome may result from more than a thousand such replications1. Recently it has been suggested2 that differentiation in a multicellular organism involves the progressive inactivation of DNA, and that, in the polytene chromosome, three conditions characterize these inactivated regions: first, inactivity with respect to RNA synthesis; secondly, late replication, replication being slower and continuing longer than over the rest of the chromosome; thirdly, the appearance of dense packing. Heavy bands and heterochromatic regions are given as typical examples of such regions.
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References
Swift, H., in The Molecular Control of Cellular Activity, 73 (McGraw-Hill, New York, 1962).
Fujita, S., Nature, 206, 742 (1965).
Whitten, J. M., Proc. Sixteenth Intern. Zool. Congress, Washington, 276 (1963).
Whitten, J. M., Science, 143, 1437 (1964).
Breuer, M. E., and Pavan, C., Chromosoma, 7, 371 (1955).
Stich, H. F., and Naylor, J. M., Exp. Cell. Res., 14, 442 (1958).
Kimoto, Y., Cytologia, 23, 478 (1958).
Kaufman, B. P., and Gay, H., The Nucleus, 1, 57 (1958).
Gay, H., Cold Spring Harb. Symp. Quant. Biol., 21, 257 (1956).
Bayreuther, K., Chromosoma, 7, 508 (1956).
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WHITTEN, J. Differential Deoxyribonucleic Acid Replication in the Giant Foot-pad Cells of Sarcophaga bullata. Nature 208, 1019–1021 (1965). https://doi.org/10.1038/2081019a0
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DOI: https://doi.org/10.1038/2081019a0
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