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Use of Galactose Oxidase in the Histochemical Examination of Mucus-secreting Cells

Nature volume 207, pages 425–426 (1965)Cite this article

Abstract

GALACTOSE oxidase is an enzyme which oxidizes the primary alcohol group of galactose or galactosamine to an aldehyde1. The enzyme attacks not only free galactose but also terminal non-reducing galactose and galactosamine units when glycosidically linked. Detection of such units in mucopolysaccharides and mucoproteins produced by mucus-secreting cells can be accomplished by staining the aldehyde produced with Schiff's reagent.

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Authors and Affiliations

  1. National Institute for Research in Dairying, Shinfield, Reading

    G. P. ROBERTS & S. K. GUPTA

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  1. G. P. ROBERTS
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  2. S. K. GUPTA
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ROBERTS, G., GUPTA, S. Use of Galactose Oxidase in the Histochemical Examination of Mucus-secreting Cells. Nature 207, 425–426 (1965). https://doi.org/10.1038/207425a0

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