Abstract
THE specificity of tritium- or carbon-14-labelled thymidine as a precursor for deoxyribonucleie acid (DNA) appears to be good in many organisms1,2. Autoradiographs examined by light and electron microscopes have demonstrated that most of the label is incorporated into nuclear chromatin during the DNA synthetic phase of cell reproduction or during an exchange process in differentiated cells3–5. Occasional evidence for the cytoplasmic incorporation of thymidine has been obtained with animal cells after treatment with deoxyribonuclease6. In egg cells and developing embryos of vertebrates there is biochemical evidence for the presence of extranuclear DNA7–9.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Reichard, P., and Estborn, B., J. Biol. Chem., 188, 839 (1951).
Friedkin, M., Tilson, D., and Roberts, D., J. Biol. Chem., 220, 521 (1956).
Taylor, J. H., Woods, P. S., and Hughes, W. L., Proc. U.S. Nat. Acad. Sci., 43, 122 (1957).
Pelc, S. R., and La Cour, L. F., Experientia, 15, 131 (1959).
Pelc, S. R., Exp. Cell Res., 29, 194 (1963).
Chèvremont, M., Chèvremont-Comhaire, S., and Baeckland, E., Arch. Biol.(Liège), 70, 811 (1959).
Hoff-Jørgenssen, E., and Zeuthen, E., Nature, 169, 245 (1952).
Bieber, S., Spence, J. A., and Hitchings, G. H., Exp. Cell Res., 16, 202 (1959).
Durland, M., Bull. Biol. France et Belgique, 95, 28 (1961).
Luft, J. H., J. Biophys. Biochem. Cytol., 2, 799 (1956).
Palade, G. E., J. Exp. Med., 95, 285 (1952).
Pelc, S. R., Coombes, J. D., and Budd, G. C., Exp. Cell Res., 24, 192 (1961).
Budd, G. C., and Pelc, S. R., Stain Technology, 39, 295 (1964).
Watson, M. L., J. Biophys. Biochem. Cytol., 4, 475 (1958).
Karnovsky, M. J., J. Biophys. Biochem. Cytol., 11, 729 (1961).
Porter, K. R., and Machado, R. D., J. Biophys. Biochem. Cytol., 7, 167 (1960).
Lund, H. A., Vatter, A. E., and Hanson, J. B., J. Biophys. Biochem. Cytol., 4, 87 (1958).
Meek, G. A., J. Roy. Micro. Soc., 81, 187 (1963).
Nass, S., and Nass, M. M. K., J. Roy. Micro. Soc., 81, 209 (1963).
Nass, M. M. K., and Nass, S., J. Cell Biol., 19, 593 (1964).
Nass, S., and Nass, M. M. K., J. Cell Biol., 19, 613 (1964).
Ris, H., and Plant, W., J. Cell Biol., 13, 383 (1962).
Ephrussi, B., J. Cell and Comp. Physiol., 52 (supp. 1), 35 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BUDD, G., MILLS, G. Labelled Cytoplasmic Organelles in Allium cepa Var. ‘White Lisbon’ after Administration of Tritiated Thymidine. Nature 205, 524–525 (1965). https://doi.org/10.1038/205524a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/205524a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
