Abstract
THE production of an antiserum to a preparation of human pituitary follicle-stimulating hormone (HP-FSH) which had been fractionated by electrophoresis on starch gel (SG4) has been described previously1,2. Immunoelectrophoresis of various HP-FSH preparations with this antiserum showed the presence of two precipitin lines. These two lines obtained with antigens of 1 mg/ml. solutions (w/v) were now investigated by comparing three HP-FSH preparations of different purity, CM 1 (ref. 3), CP 1 (ref. 3) and SG 4 (Fig. 1, A, B, C), and by absorbing the antiserum with various proteins.
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References
Butt, W. R., Crooke, A. C., Cunningham, F. J., and Wolf, Annelisese, J. Endocrinol., 25, 541 (1963).
Wolf, Anneliese, Nature, 198, 1308 (1963).
Butt, W. R., Crooke, A. C., and Cunningham, F. J., Biochem. J., 81, 596 (1961).
Hartree, Anne S., Butt, W. R., and Kirkham, K. E., J. Endocrin., 29, 61 (1964).
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WOLF, A. Antigenic Components in Human Pituitary Follicle-stimulating Hormone Preparations of Various Purities. Nature 205, 504–505 (1965). https://doi.org/10.1038/205504a0
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DOI: https://doi.org/10.1038/205504a0
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